Chimeric genes as dominant selectable markers in plant cells

Binary vectors which allow the exchange of plant selectable markers and reporter genes

With the binary vectors pBIG and pBIB it is possible to: i) exchange the plant selectable marker/promoter fusion and the reporter gene, ii) use one vector and Agrobacterium strain system, thus reducing variables associated with using different vectors and strains, iii) allow for divergent transcription of the selectable marker gene and of promoter/reporter gene fusions. The two plasmids are der...

Recycling selectable markers in mouse embryonic stem cells.

As a result of gene targeting, selectable markers are usually permanently introduced into the mammalian genome. Multiple gene targeting events in the same cell line can therefore exhaust the pool of markers available and limit subsequent manipulations or genetic analysis. In this study, we describe the combined use of homologous and CRE-loxP-mediated recombination to generate mouse embryonic st...

Selectable Markers for Gene Therapy

One of the major problems with current approaches to gene 9 therapy is the instability of expression of genes transferred into 10 recipient cells. Although in theory, homologous recombination 11 or use of artificial chromosomes can stabilize sequences with 12 wild-type regulatory regions, such approaches to gene therapy 13 are not yet feasible and may not be efficient for some time to 14 come. ...

Comparison of two alternative dominant selectable markers for wine yeast transformation.

Genetic improvement of industrial yeast strains is restricted by the availability of selectable transformation markers. Antibiotic resistance markers have to be avoided for public health reasons, while auxotrophy markers are generally not useful for wine yeast strain transformation because most industrial Saccharomyces cerevisiae strains are prototrophic. For this work, we performed a comparati...

Cloning and Mutagenesis of the for Resistance to Pseudomonic Acid by Generation of Dominant Selectable Markers